海桐皮方凝胶贴膏对大鼠膝骨关节炎作用机制OA

Objective:To explore therapeutic effect of the Haidongpi formula gel plaster on knee osteoarthritis(KOA)in rats and the molecular mechanism of regulating the SOCS3/JAK/STAT signaling pathway.Methods:36 8-week-old SPF-grade SD rats were randomly divided into blank group,sham operation group,KOA control group,and treatment group.KOA rat model was established by the Panicker method.The treatment group was treated with Haidongpi formula gel plaster externally,while the KOA control group was treated with blank plasters.The inter-vention lasted for two courses.The mechanical pain threshold(PWT),thermal radiation pain threshold(PWL),and knee joint diameter(KD)of the rats were evaluated by behavioral tests.The levels of IL-1β,IL-6,and TNF-αin ser-um were detected by ELISA.The morphology of cartilage was observed by HE and safranin O-fast green staining.The expression levels of SOCS3,COX-2,JAK2,and STAT3 genes and related proteins in cartilage tissue were detec-ted by RT-qPCR and Western blot.Results:Behavioral tests showed that after 2 weeks of intervention with the Haid-ongpi formula gel plaster,PWL of the treatment group rats recovered,and there was no statistically significant differ-ence compared with the blank group(P>0.05).The PWT showed continuous upward trend,and the degree of knee joint swelling recovered faster than that of the KOA control group.ELISA indicated that the levels of IL-1β,IL-6,and TNF-αin the serum of the treatment group were lower than those in the KOA control group(P<0.01).Histological staining showed that after intervention with the Haidongpi formula gel plaster,surface of the cartilage became smoot-her,structural integrity improved,arrangement of chondrocytes became regular,and safranin O staining recovered significantly,with less loss of proteoglycans.Molecular biological detection showed that the expression of SOCS3 gene and protein in the treatment group was higher than that in the KOA control group,the expression of COX-2 was significantly decreased,and the ratios of p-JAK2/JAK2 and p-STAT3/STAT3 decreased(P<0.01).Conclusion:Haidongpi formula gel plaster can relieve pain and joint swelling in KOA rats and protect joint cartilage.The mecha-nism may be related to the upregulation of SOCS3 expression,enhancing the negative regulation of the JAK/STAT signaling pathway,and inhibiting the release of downstream pro-inflammatory factors.