不同能量和蛋白质水平饲粮添加抗菌肽对肉鸡生产性能及免疫功能的影响OA
Effects of Dietary Antimicrobial Peptide Supplementation at Graded Energy and Protein Levels on Production Performance and Immune Function in Broilers
研究旨在探究不同蛋白质(A)、能量(B)水平饲粮中添加抗菌肽(C)对肉鸡生产性能及免疫功能的影响.选取480只1日龄817杂交肉仔鸡,随机分为8个处理组,每组6个重复,每个重复10只鸡.试验分为前期(1～21 d)和后期(22～42 d)两个阶段,采用2×2×2的3因素析因设计,设置A、B、C 3因素,A分为低蛋白组(A1,前期19%、后期17%)和高蛋白组(A2,前期21%、后期19%),B分为低能量组(B1,前期12.18 MJ/kg、后期12.81 MJ/kg)和高能量组(B2,前期12.60 MJ/kg、后期13.23 MJ/kg),C分为0添加组(C1,抗菌肽添加量为0)和200 mg/kg添加组(C2,抗菌肽添加量为200 mg/kg).结果表明:3因素交互作用显著影响1～21 d平均日采食量(P<0.05).高蛋白低能量组(A2B1)在22～42 d的平均日增重显著高于高蛋白高能量组(A2B2)(P<0.05);低能量组(B1)1～42 d平均日增重显著高于高能量组(B2)(P<0.05);A×B交互作用显著提升了高蛋白低能量组(A2B1)胸肌率(P<0.05);添加抗菌肽显著提高了半净膛率(P<0.05),并显著升高了高能量组的腹脂率(P<0.05).低蛋白组肝脏指数显著高于高蛋白组(P<0.05);抗菌肽显著降低了血清促炎因子[肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)]含量(P<0.05),提升抗炎因子[白细胞介素4(IL-4)、白细胞介素10(IL-10)]水平(P<0.05);A×B×C交互作用表明,低蛋白高能量+200 mg/kg抗菌肽添加组(A1B2C2)促炎因子水平最低,而高蛋白低能量+200 mg/kg抗菌肽添加组(A2B1C2)免疫平衡最优,兼具高免疫球蛋白A(IgA)与低TNF-α水平.综上所述,高蛋白低能量饲粮添加200 mg/kg抗菌肽可提升肉鸡生长性能、屠宰性能及免疫功能.
The experiment was conducted to investigate the effects of dietary protein(A),energy(B)levels,and antimicrobial peptide supplementation(C)on production performance and immune function in broilers.A total of 480 one-day-old 817 hybrid broilers were randomly allocated to eight treatment groups,each com-prising six replicates with ten broilers per replicate.The experiment was divided into two stages:the early stage(1-21 d)and the late stage(22-42 d),employing a 2×2×2 factorial design with three factors(A,B,and C).Factor A was categorized into a low-protein group(A1,19%in the early stage and 17%in the late stage)and a high-protein group(A2,21%in the early stage and 19%in the late stage).Factor B was divided into a low-energy group(B1,12.18 MJ/kg in the early stage and 12.81 MJ/kg in the late stage)and a high-energy group(B2,12.60 MJ/kg in the early stage and 13.23 MJ/kg in the late stage).Factor C consisted of a zero-addition group(C1,the addition amount of antimicrobial peptide was 0)and a 200 mg/kg addition group(C2,the addition amount of antimicrobial peptide was 200 mg/kg).The results indicated significant three-way interactions(A×B×C)on average daily feed intake during days 1-21(P<0.05),while the A2B1 group demonstrated higher average daily gain than A2B2 during days 22-42(P<0.05).The average daily gain of low-energy group was significantly higher than that of high-energy group during the experimental period(P<0.05).For slaughter traits,A×B interactions enhanced pectoral muscle rate in the A2B1 group(P<0.05),with antimicrobial peptides increasing the semi-eviscerated weight rate and increasing abdominal fat rate in the high-energy group(P<0.05).The liver index of low protein group was significantly higher than that of high protein group(P<0.05).Antimicrobial peptides significantly decreased the contents of serum pro-inflammatory factors(TNF-α,IFN-γ)and increased the levels of anti-inflammatory factors(IL-4,IL-10)(P<0.05).The A×B×C interaction indicates that,the A2B1C2 combina-tion achieved optimal immune balance through high IgA and low TNF-α levels,whereas A1B2C2 exhibited minimal inflammatory responses.In conclusion,adding 200 mg/kg antimicrobial peptides to high protein and low energy diets can improve the growth performance,slaughter performance and immune function of broilers.
董永琪;王丽君;赵祥民;张亚军;禹永利;秦士贞;唐德富
甘肃农业大学动物科学技术学院,甘肃 兰州 730070甘肃农业大学动物科学技术学院,甘肃 兰州 730070甘肃农业大学动物科学技术学院,甘肃 兰州 730070甘肃农业大学动物科学技术学院,甘肃 兰州 730070甘肃农业大学动物科学技术学院,甘肃 兰州 730070甘肃农业大学动物科学技术学院,甘肃 兰州 730070甘肃农业大学动物科学技术学院,甘肃 兰州 730070
农业科技
肉鸡能量蛋白质抗菌肽生产性能免疫功能
broilersenergyproteinantimicrobial peptidesproduction performanceimmune function
《饲料工业》 2026 (9)
47-57,11
甘肃省高校产业支撑计划项目[2023CYZC-47]
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