功能真菌-除杂磷石膏复合改良剂在滨海盐碱土中的修复应用OA
Application of fungus-purified phosphogypsum composite conditioner in coastal saline-alkali soils
滨海盐碱土中的高pH和高盐导致土壤有效磷严重缺乏,制约了生态恢复和耕地扩容.本研究以如东滨海中度盐渍土为对象,通过液体摇瓶实验优选溶磷真菌黑曲霉(CCTCC M2023240),并设置6个处理(CK、PG、ANG、ANG+PG、NS、NS+PG),在室内恒温培养30 d,系统评价功能真菌与除杂磷石膏联用对土壤pH、土壤有效磷含量及微生物活性的影响.结果表明:该真菌在NaCl质量分数为0~8%时呼吸强度持续升高,在16%的NaCl环境下丧失活性.在盐胁迫环境下能稳定产酸溶磷.在土壤培养试验中,除杂磷石膏(PG)和黑曲霉(ANG)的添加使土壤pH显著降低.同时,除杂磷石膏的添加让土壤有效磷含量显著提高.真菌-磷石膏(ANG+PG)复合处理pH最低达7.29,有效磷含量为31.8 mg/kg,与单独施用除杂磷石膏无显著差异.磷石膏提供的养分提高了黑曲霉的呼吸强度,并延长其存活时间,这使得黑曲霉能更持久改良滨海盐碱土壤,说明了黑曲霉-除杂磷石膏复配土壤改良剂在治理滨海盐碱土壤的应用潜力.此外,通过扫描电子显微镜(SEM)发现,黑曲霉在滨海盐碱土壤中能形成稳定菌丝结构,有利于团聚体形成,促进盐碱土壤的改良.本研究通过黑曲霉-除杂磷石膏复合土壤改良剂降低盐碱度并提升磷有效性,为滨海盐碱地改良与固废磷石膏高值利用提供理论依据与技术原型.
High pH and salinity in coastal saline-alkali soil cause severe deficiencies in available phosphorus,hindering ecological restoration and farmland expansion.Herein,we used moderately saline coastal soils in Rudong to evaluate the combined effects of functional fungus and phosphogypsum on soil pH,soil available phosphorus,and microbial activity.Phosphorus-solubilizing fungi(CCTCC M2023240)was optimized through liquid shake flask experiments.Six treatments(CK,PG,ANG,ANG+PG,NS,NS+PG)were established and cultured at a constant temperature for 30 days indoors.As a result,the fungus exhibited continuously increasing respiratory intensity within the 0-8%NaCl range but lost activity at 16%salinity.More importantly,organic acids were constantly produced to solubilize phosphorus under saline stress.In soil culture tests,the addition of phosphogypsum(PG)and Aspergillus niger(ANG)could significantly lower the soil pH.Meanwhile,phosphogypsum addition markedly increased soil available phosphorus content.Notably,the fungal-phosphogypsum(ANG+PG)composite treatment led to the lowest soil pH of 7.29,with available phosphorus at 31.8 mg/kg,indicating no significant difference compared to the application of purified phosphogypsum alone.However,the nutrients from phosphogypsum enhanced the respiratory intensity of Aspergillus niger and prolonged its survival time.According to scanning electron microscopy(SEM),Aspergillus niger could grow in saline-alkali soil and survive under such conditions,suggesting its potential for saline-alkali soil remediation.Our research work demonstrated that the Aspergillus niger-phosphogypsum composite soil conditioner could reduce salinity and enhance phosphorus availability,and it will provide a theoretical basis and technological prototype for coastal saline-alkali land remediation and high-value utilization of solid waste phosphogypsum.
姜明禺;李真;陈月坤;黄韵洁;李思琦;王萍;袁紫仪;王硕;江建华;戴虹
南京农业大学资源与环境科学学院,江苏南京 210095南京农业大学资源与环境科学学院,江苏南京 210095||江苏省有机固体废弃物利用重点实验室,江苏 南京 210095江苏省南通外向型农业综合开发区农村工作局,江苏 南通 226409南京农业大学资源与环境科学学院,江苏南京 210095南京农业大学资源与环境科学学院,江苏南京 210095江苏省南通外向型农业综合开发区农村工作局,江苏 南通 226409江苏省南通外向型农业综合开发区农村工作局,江苏 南通 226409江苏省南通外向型农业综合开发区农村工作局,江苏 南通 226409江苏省南通外向型农业综合开发区农村工作局,江苏 南通 226409江苏省南通外向型农业综合开发区农村工作局,江苏 南通 226409
资源环境
盐碱地磷石膏真菌土壤改良剂
saline soilphosphogypsumfungisoil conditioner
《生物加工过程》 2026 (2)
152-159,8
南通外向型农业综合开发区(苴镇街道)盐碱耕地改良利用项目国家重点研发计划(2023YFC3707600)
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