基于常压室温等离子体(ARTP)诱变的胞嘧啶高产菌株筛选及其发酵条件优化OA

Cytosine is widely applied in pharmaceutical and agricultural fields.Traditional chemical synthesis methods are often accompanied with high environmental pollution,exorbitant costs,and complex purification processes,so microbial fermentation has emerged as a viable alternative due to its environmental friendliness and sustainability.In this study,a high-yielding mutant strain(E.coli Cyto-85)was developed from the parental strain E.coli through atmospheric and room-temperature plasma(ARTP)mutagenesis,and the corresponding fermentation process was subsequently optimized.Based on our single-factor experiments and Box-Behnken design results,the optimal fermentation process was conducted with glucose supplementation at 49.4 g/L,peptone supplementation at 21.1 g/L,and initial pH 7.12,leading to cytosine production of 1.43 g/L.This research work established a robust microbial platform for green biosynthesis of cytosine,and could further provide fermentation strategies to enhance its industrial-scale production.