食品中片球菌属鉴定方法比较分析OA

In this study,the traditional physiological and biochemical method,a VITEK automatic biochemical analyzer,the analytical profile index(API)biochemical identification system,real-time fluorescence quantitative polymerase chain reaction(real-time PCR)and matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS)were used comparatively to identify the species of Pediococcus spp.in food.The results showed that the traditional physiological and biochemical method was the most accurate but time-consuming and labor-intensive;the VITEK automatic biochemical analyzer gave unstable results as the database states that Pediococcus acidilactici is negative for amygdalin reaction,whereas some strains actually test positive;the API biochemical identification system yielded accurate results at 24 h of culture,while deviations occurred when the culture period was extended to 48 h;real-time PCR was time-saving,accurate,and suitable for popularization;the MALDI-TOF MS method had the shortest operation time but high equipment requirements,making it difficult to popularize in primary-level institutions.In summary,the traditional physiological and biochemical method,real-time PCR,and MALDI-TOF MS can all serve as standard methods for the identification of Pediococcus spp.in food.The results of this study can provide a technical basis for the formulation of relevant national standards.