高效液相色谱法检测液态乳中5-羟甲基糠醛与糠醛含量OA

A high-performance liquid chromatography method was developed to determine the contents of 5-hydroxymethylfurfural and furfural in liquid milk.The samples were extracted with oxalic acid solution in a boiling water bath,then the protein and fat were precipitated by adding trichloroacetic acid solution.The supernatant was separated on an XDB-C18 column(4.6 mm×250 mm,5 μm)using 10%acetonitrile solution as the mobile phase.The analytes were detection with an ultraviolet detector and quantified by the external standard method.The experimental results showed that the correlation coefficient of 5-hydroxymethylfurfural was 0.999 8,with a spiked recovery ranging from 84.6%to 94.3%,a limit of detection(LOD)of 0.005 mg/kg,and a coefficient of variation(CV)of 0.15%to 0.36%.For furfural,the correlation coefficient was 0.999 4,with a spiked recovery rate ranging from 73.7%to 92.0%,a LOD of 0.005 mg/kg,and a CV of 0.95%to 1.54%.This method is characterized by simple operation,low cost,and excellent separation performance,and it is suitable for the quantitative analysis of 5-hydroxymethylfurfural and furfural in liquid milk,providing technical support for the quality control and process optimization of dairy products.