基于微流控技术的ELISA检测器件常见衬底蛋白孵育方法的探究及应用OA
Exploration and application of common substrate protein incubation methods for ELISA detection device based on microfluidic technology
为研究微流控器件中常用衬底的蛋白吸附能力,使微流控技术更好地应用于酶联免疫吸附测定(ELISA)优化中,进一步综合优化检测时间、成本及所需样本量,该文探究并总结了加强玻璃衬底及铌酸锂晶片蛋白吸附能力的方法,结果表明 3-氨丙基三乙氧基硅烷(3-APTES)可以提高衬底表面氨基数目,通过缩水反应促进衬底与蛋白质的结合.在此基础上,该文提出了基于液滴的 ELISA 检测方法,将经过3-APTES 处理后的玻璃衬底与微液滴相结合,在体声波驱动的微液滴混合下完成 SARS-CoV-2(2019-nCoV)抗体检测,使所需样品量减少了 95%,将抗体孵育时间缩短为40 s,揭示了微流控器件在免疫检测中的应用潜力.
In order to study the protein binding ability of substrates commonly used in microfluidic devices,to optimize enzyme-linked immunosorbent assay(ELISA)with microfluidic technology and further optimize assay time,cost and sample size required,this paper explores and summarizes the methods to enhance the protein binding ability of glass substrates and lithium niobate wafers,and the results show that 3-aminopropyl triethoxysilane(3-APTES)can increase the number of amino acids on the surface of substrates and promote the binding of substrates to proteins.On this basis,this paper proposes a droplet-based ELISA detection method that combines the 3-APTES-treated glass substrate with microdroplets to accomplish SARS-CoV-2(2019-nCoV)antibody detection with the assistance of acoustic streaming,which reduces the amount of required samples by 95%,reducing the antibody incubation time to 40 s,revealing the potential of microfluidic devices for immunoassay applications.
王琪;宋建超
南京理工大学 微电子学院,江苏 南京 210094南京理工大学 微电子学院,江苏 南京 210094
信息技术与安全科学
酶联免疫吸附测定微流控衬底处理蛋白吸附免疫检测
enzyme-linked immunosorbent assaymicrofluidicssubstrate treatmentprotein bindingimmunoassay
《南京理工大学学报（自然科学版）》 2026 (2)
143-151,9
国家自然科学基金(62531002)中央高校基本科研业务费专项资金(309250106053092501054030924012001)
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