抑郁症慢性不可预知温和刺激动物模型分析及评价OA

Objective To analyze the modeling characteristics of chronic unpredictable mild stimulation(CUMS)animal models of depression in China,and explore depression models that are more suitable for the disease.Methods Using"CUMS"and"animal models"as keywords,search for relevant literature on animal experiments from the China National Knowledge Infrastructure database until May 2024,and summarize animal species,modeling methods,detection indicators,etc.After one week of adaptive feeding,male SD rats were randomly divided into a control group and a CUMS model group.The two groups were treated according to different modeling methods,and after 21 days of modeling,relevant behavioral tests and hippocampal HE staining were performed.Results A total of 332 standard literature were included,with male animals being the majority,including SD rats(226 times,68.07%),Wistar rats(40 times,12.05%),and C57BL/6J mice(30 times,9.04%);The main molding cycles are 21 days(75 times),42 days(67 times),and 28 days(65 times).The success of modeling is mainly based on animal body mass,behavior,molecular testing,etc.The pathology was mostly hippocampal HE staining.After 21 days of CUMS combined with solitary confinement,the model group showed decrease in body weight,food intake,sucrose preference,and total distance of open field compared to the control group.The number and duration of forced swimming and floating increased(P<0.05).Hippocampal tissues were subjected to hematoxylin and eosin(HE)staining for pathological morphological evaluation.Conclusion The CUMS combined with solitary care model showed a decrease in body weight,food intake,sucrose preference,and total open field distance,with an increase in resting time,and the modeling was basically successful.