基于转录组学探究金鱼早期发育过程中脂代谢相关基因的表达OA

To elucidate the expression dynamics of lipid metabolism-related genes during early development in goldfish(Carassius auratus),we conducted transcriptome sequencing to profile differentially expressed genes(DEGs)at three critical developmental stages(0 h,48 h,and 5 d post-fertilization)in two distinct goldfish varieties(Oranda and Pearl-scale).Subsequent qRT-PCR validation confirmed the transcriptomic findings.Comparative analysis revealed a significant number of DEGs across all developmental transitions in both varieties.Enrichment analysis indicated that downregulated DEGs(48 h vs 0 h,5 d vs 48 h,and 5 d vs 0 h)were predominantly associated with pathways involved in RNA trans-port,pyrimidine metabolism,and oocyte meiosis.In contrast,upregulated DEGs exhibited pathway enrichment exclu-sively in the 5 d vs 48 h comparison,with both varieties showing significant enrichment in the PPAR signaling pathway.Further analysis identified twelve candidate genes including apoba,fads2,pparα,and pparγ as potential key regulators of lipid metabolism during early goldfish development.Collectively,these findings demonstrate pronounced differences in PPAR signaling and lipid metabolism-related gene expression between the 5 d and 48 h stages,supporting the hypothesis that post-hatching goldfish undergo a marked enhancement in lipid metabolic capacity.