副溶血弧菌噬菌体VpP2326的分离鉴定及表征OA

To promote the development of phage-based technologies for the prevention and control of Vibrio parahae-molyticus,a phage was isolated and purified by the double-layer agar plate method with V.parahaemolyticus RIMD2210633 strain as the host bacterium.Thereafter,its biological characteristics were explored.The results showed that the isolated phage VpP2326 had an icosahedral head and a short tail.It exhibited a significant antibacterial effect on the host bacterium and a strong host specificity.The optimal multiplicity of infection(MOI)of this phage was 0.1,the incubation period was less than 5 min,and the burst size was approximately 147 PFU·cell-1.VpP2326 had good thermal and pH stabili-ty,was sensitive to chloroform and proteases,and was highly sensitive to UV radiation.The nucleic acid type of this phage was double-stranded DNA,the whole genome was approximately 43 kb,with the G+C content of 49.37％,46 open reading frames are predicted in the genome.This study laid the foundation for the development and application of VpP2326.