超甜玉米籽粒维生素C含量关联位点定位及基因挖掘OA

To uncover the regulatory mechanisms underlying the accumulation of nutrients in the kernels of super-sweet corn,this study employed the BLINK model to identify the major loci regulating vitamin C content in super-sweet corn kernels and predict the candidate genes.The results showed that the vitamin C content in super-sweet corn kernels ranged from 0.156 mg/g to 1.289 mg/g,with an average of 0.749 mg/g,exhibiting a normal distribution.A genome-wide association study(GWAS)identified six single nucleotide polymorphism(SNP)loci significantly associated with kernel vitamin C content,with the phenotypic variation explained(PVE)ranging from 9.53%to 12.83%,among which the Affx-91328273 locus had the highest PVE.A total of 153 candidate genes were identified within the 200 kb confidence interval upstream and downstream of the significant SNP loci,of which 58 were annotated genes,accounting for 37.9%.Six genes including GRMZM2G093098,GRMZM2G106061,GRMZM2G179308,GRMZM2G101274,GRMZM2G004222 and GRMZM2G133631 were identifed as key genes regulating kernel vitamin C content in super-sweet corn.These genes were mainly involved in cell division,signal trans-duction,energy metabolism and other pathways.These findings provide important support for an in-depth understanding of the genetic mechanisms underlying kernel vitamin C content in super-sweet corn.