OsCERK1DY基因导入籼稻中与丛枝菌根(AM)真菌的共生效应OA

In this study,using the indica rice restorer line Chunhui 350 as the control and the single-segment substi-tution line Chunhui 356,which carries the arbuscular mycorrhizal(AM)fungus high-efficiency symbiosis gene Os-CERK1DY,as the experimental material,four phosphorus(P)fertilizer application levels were set:0 kg/hm2(P0),50%of the conventional phosphorus application rate(P50),75%of the conventional phosphorus application rate(P75),and the conventional phosphorus application rate(P100,with P2 O5 application at 75 kg/hm2).The symbiotic effect of the OsCERK1DY gene with arbuscular mycorrhizal(AM)fungi in indica rice was evaluated.The results showed that,compared with Chunhui 350,the AM fungal colonization rate in the roots of Chunhui 356 was signifi-cantly increased.Overall,under different phosphorus fertilizer levels in the field,its chlorophyll content,dry matter weight,phosphorus uptake,and phosphorus fertilizer use efficiency were higher than those of Chunhui 350.Results of the two-year field experiment showed that the effective panicle number of Chunhui 356 was significantly higher than that of Chunhui 350 under all phosphorus fertilizer levels(P<0.05).Under the three treatments P0,P50,and P75,the actual yield of Chunhui 356 was significantly higher than that of Chunhui 350(P<0.05).Analysis of the rice rhizosphere soil mi-crobial diversity showed that,compared with Chunhui 350,the rhizosphere soil of Chunhui 356 exhibited higher species richness and diversity,with enrichment of more bacteria from the genera Chryseobacterium and Bradyrhizobium.The relative abundances of functional genes related to hydrocarbon degradation and nitrogen fixation were significantly(P<0.05)or ex-tremely significantly(P<0.01)increased.In conclusion,introducing the OsCERK1DY gene into indica rice can produce beneficial symbiotic effects and can be used to breed rice cultivars with high nutrient use efficiency.