基于cAMP/PKA/CREB通路探讨红花化瘀汤减轻肌筋膜疼痛综合征模型大鼠炎性损伤作用及机制OA

Objective:To investigate the mechanism by which Honghua Huayu Decoction alleviates inflammatory damage in myofascial pain syndrome(MPS).Methods:Male SD rats were randomly divided into control group,model group,celecoxib group,HHD group,and HHD plus protein kinase A(PKA)inhibitor H-89 group.An electromyograph was used to measure spontaneous motor activity frequency;a paw pressure analgesia meter to assess the pain threshold at trigger points;ELISA to detect serum levels of substance P(SP),calcitonin gene-related peptide(CGRP),interleukin-1 β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-10(IL-10)levels;HE staining to detect pathological changes in muscle tissue;Western blot to detect the expression of CD68 and CD206 proteins in muscle tissue,as well as the p-PKA/PKA and p-cAMP response element-binding protein(CREB)/CREB ratios;and ELISA to detect cyclic adenosine monophosphate(cAMP)levels in muscle tissue.Results:Compared with the control group,the model group exhibited increased spontaneous electrical activity frequency,SP,CGRP,CD68,IL-1β,IL-6,and TNF-α(P＜0.05),decreased trigger point pain thresholds,CD206,IL-10,cAMP,p-PKA/PKA,and p-CREB/CREB(P＜0.05),and pathological changes occurred in the muscle tissue;both celecoxib and HHD significantly improved the aforementioned pathological indicators in rats with MPS(P＜0.05);the therapeutic effect of HHD was superior to that of the celecoxib group(P＜0.05);H-89 partially reversed the effects of HHD(P＜0.05).Conclusion:Honghua Hua;Decoctic may alleviate inflammatory and pain responses in rats MPS by influencing the expression of proteins associated with macrophage phenotypes,and its mechanism is related to the activation of the cAMP/PKA/CREB signaling pathway.