3D打印载米诺环素nHA/CS复合支架修复犬种植体周围炎伴骨缺损OA

Objective:To explore the repair effect of 3D-printed nano-metronidazole hydroxyapatite(nano-hydroxyapatite,nHA)/chitosan(Chitosan,CS)composite scaffolds on periodontal disease with bone defect in dogs.Methods:A total of 6 male beagle dogs had their posterior mandibular teeth immediately implanted.After 4 weeks,a periodontal disease model was estab-lished around the implants using the suture ligation method,and the scaffold material was reimplanted.The bilateral mandibles of dogs were divided into control C1(nHA)group,control C2(minocycline)group,experimental T0(nHA/CS)group and exper-imental T1(nHA/CS/minocycline)group.Analysis of stent effects on beagle dogs with peri-implantitis bone defects using com-puterized tomography(CBCT)and histopathology.And the levels of IL-8 and IL-34 in the peri-implant gingival crevicular fluid were measured by ELISA on 3rd,7th and 30th day after immediate implantation and stent reimplantation.Results:After stent re-implantation,ELISA test showed that the IL-8 and IL-34 levels in the experimental T1 group were lower than the experimental T0 group at each measurement time point,and the IL-8 and IL-34 levels were lower than the control C2 group at 30th day after surgery,showing a statistically significant difference(P<0.05).CT showed no obvious resorptive shadow in the experimental T1 group in the implant 1 month after surgery,while bone defect was still present in the other groups.DD,DW and BL values were significant in each group(F=19.232,216.008,71.985,P<0.05).The histological staining of H&E showed the best effect of the experimental T1 bone composition,and a large number of fibroblastic vessels and osteoblasts were obviously biodegradable.Con-clusion:3D printed nHA/CS carrier Minocycline composite scaffold shows a promising repair effect on canine implant-related peri-odontitis with bone defects.That has a good antibacterial effect and can effectively promote the repair of alveolar bone defects.