膝关节创伤性滑膜炎兔模型的复制与评价OA

Objective To replicate the rabbit model of traumatic synovitis of knee joint using micro-damage combined with electrical stimulation and to evaluate the model.Methods Eighteen New Zealand white rabbits were numbered by body weight and divided into a sham-operated group,an electrical stimulation group,and a combina-tion group using stratified randomization,with 6 rabbits per group(half male and half female).The electrical stimula-tion group was modeled using the classical electrical stimulation method,while the combination group was modeled using micro-damage combined with electrical stimulation.Changes in knee circumference and skin temperature around the knee were measured.After modeling,synovial tissue and joint fluid from the knee joint were collected.The levels of inflammatory factors interleukin(IL)-1 β and IL-18 in the joint fluid were detected,and pathological chan-ges in the synovial tissue were observed.Results Compared with the sham-operated group,the knee circumference,skin temperature,and the levels of IL-1β and IL-18 in the joint fluid were significantly increased in the electrical stimulation and combination groups after modeling,with statistically significant differences(P＜0.05 or P＜0.01).In-flammatory infiltration was observed under microscopy in the synovial tissue of the knee joint.Compared with electri-cal stimulation alone,the combination of micro-damage and electrical stimulation induced a more rapid increase in knee circumference and skin temperature,more severe inflammatory pathological changes in the synovial tissue,and higher levels of IL-1β and IL-18 in the joint fluid(P＜0.01).Conclusion The combination of micro-damage and electrical stimulation can successfully and efficiently replicate a rabbit model of traumatic synovitis of knee joint,in-ducing inflammatory changes in the synovial tissue,knee swelling,redness,and elevated skin temperature,which aligns with the pathogenic characteristics of traumatic synovitis of knee joint.