决明子醇提物对金黄色葡萄球菌的体外抑菌作用研究OA

[Objective]To investigate the in vitro antibacterial activity of alcohol extract from cassia seed.[Methods]Al-cohol extracts of Cassia obtusifolia were prepared at concentrations of 0.25,0.50,0.75,1.00,1.50,and 2.00 g/mL.The in-hibition zone diameters against Staphylococcus aureus were determined using the filter paper method and the Oxford cup meth-od,with sterile water as a blank control.[Results]The alcohol extract of Cassia obtusifolia exhibited significant antibacterial activity against Staphylococcus aureus in a concentration-dependent manner.The strongest antibacterial effect was observed at the concentration of 2.00 g/mL,with inhibition zone diameters of 25.6 mm(filter paper disc method)and 28.4 mm(Oxford cup method).The weakest effect was observed at 0.25 g/mL,with inhibition zone diameters of 13.6 mm and 13.4 mm,respectively.The Oxford cup method showed superior antibacterial effects compared to the filter paper method.[Conclusion]The alcohol extract of Cassia obtusifolia possesses good in vitro antibacterial activity against Staphylococcus aureus,and the antibacterial activity is positively correlated with the extract concentration.These findings provide a preliminary scientific basis for the integrated utilization of Cassia obtusifolia in ecological fruit tree cultivation and fruit food safety.