圆锥茎阿魏乙酸乙酯部位化学成分及葡萄糖摄取活性检测OA

Using a combination of multiple column chromatography and high-performance liquid chromatography separation techniques,the ethyl acetate fraction of Ferula conocaula Korovin was isolated and purified.Modern spectroscopic methods were employed to structurally characterize the obtained monomeric compounds.This study successfully isolated 19 monomeric compounds from the ethyl acetate fraction of Ferula conocaula Korovin.including 4 polyphenolic monomers,14 caffeoylquinic acid monomers,and 1 flavonoid monomer.They are identified as caffeic acid(1),caffeic acid methyl ester(2),3,4-dihydroxybenzaldehyde(3),5-O-caffeoylquinic acid(4),1-O-caffeoylquinic acid(5),chlorgenic acid(6),methyl chlorogenate(7),3-O-trans-feruloylquinic acid(8),methyl 5-O-p-coumaroylquinic acid(9),methyl 5-O-feruloylquinate(10),acanthopacid G(11),1-(3,4-dihydroxycinnamoyl)cyclopentene-2,3-diol(12),4,5-di-O-caffeoylquinic acid 1-methyl ether(13),4-O-caffeoyl-5-O-feruloylquinic acid methyl ester(14),methy 3,5-dicaffeoylquinate(15),1,5-di-O-caffeoylquinic acid(16),1,3-di-O-caffeoylquinic acid(17),methyl 4-O-E-feruloyl 5-O-E-caffeoyl-quinate(18),and luteolin 7-O-glucoside(19).It is worth nothing that compounds 1-6 and 8-19 were isolated from this plant for the first time.Glucose uptake activity assay experiments showed that compounds 7,13,15,and 16 had strong blood glucose lowering effects.