代谢组学探讨布渣叶干预高脂血症小鼠降脂作用及精氨酸-脯氨酸-多胺代谢轴调控机制OA

This study employed a high-fat diet(HFD)-induced hyperlipidemia mice model to system‑atically evaluate the lipid-lowering and hepatoprotective effects of Microctis Folium and to elucidate its regulatory mechanism on the arginine-proline-polyamine metabolic axis using integrated untarget‑ed and targeted metabolomics.Serum untargeted metabolomics was performed using UPLC-QTOF-MS/MS to screen for differential metabolites and conduct pathway enrichment analysis.Subsequently,targeted metabolomics based on UPLC-TQ-MS/MS was employed to quantify key metabolites includ‑ing L-arginine,proline,creatine,and polyamines(spermidine and spermine).Microctis Folium significantly reduced TC,TG,and LDL-C levels and decreased ALT and AST while increasing HDL-C across dose groups.Untargeted metabolomics revealed broad metabolic disturbances induced by HFD,with significant enrichment of arginine and proline metabolism;differential metabolites mainly involved amino acids and derivatives,polyamines,creatine-related metabolites,as well as lipids and organic acids.Microctis Folium treatment globally reversed these metabolic abnormalities.Targeted quantification further confirmed that L-arginine,proline,creatine,and polyamines(spermine/spermidine)were decreased in the model group,and Microctis Folium markedly restored their levels toward those of controls.Collectively,Microctis Folium exhibits pronounced lipid-lower‑ing and hepatoprotective activities,which may be mediated by modulation of the arginine-proline-polyamine metabolic axis and associated metabolic remodeling of nitrogen and energy metabolism,thereby promoting restoration of metabolic homeostasis.These findings provide metabolomics-based evidence for the mechanism of action of Microctis Folium and support future screening of its bioactive constituents and translational development.