肌萎缩侧索硬化症患者血液外泌体的苏木化修饰研究OA

The clinical diagnosis of ALS lacks early biomarkers,and the complexity of peripheral blood components makes it difficult to directly reflect pathological changes.Blood exosomes,which stably carry protein information from their source cells,provide a window for non-invasive disease monitoring.SUMOylation is a crucial post-translational modification that plays a key regulatory role in disease.However,the characteristics of SUMO-1 modification in the blood exosomes of ALS pa‑tients remain unclear.To address this,this study utilized an affinity ligand(CP-1)screened via phage display technology to synthesize a specific enrichment material(M2).Combined with LC-MS/MS,a proteomic analysis of SUMO-1 modifications was performed on blood exosomes from 4 early-stage and 4 mid-to-late-stage ALS patients.A total of 119 SUMO-1-modified proteins were identi‑fied.The overall abundance of these modified proteins was significantly higher in middle-to-late-stage patients compared to early-stage patients,and they were primarily enriched in the chemokine signal‑ing and HIF-1 signaling pathways.These findings suggest that exosomal SUMO-1 modification may be involved in the pathological process of ALS,providing new clues for understanding the disease mechanism.