NAC转录因子家族在金银花黄花和白花中的差异分析OA

Taking the transcriptome data of yellow and white flowers of the honeysuckle variety'Zhengyinhua 3'as experimental materials,bioinformatics analysis methods were employed to study the expression patterns and potential regulatory mechanisms of NAC genes in different flower colors(yellow and white),in order to reveal their roles in honeysuckle flower color formation and provide references for further research on the NAC gene family in honeysuckle.The results showed that the NAC transcription factor family in this honeysuckle variety had 58 members,with amino acid numbers ranging from 44 to 737 and an average relative molecular weight of 38 347.79 Da.The majority of NAC transcription factors were acidic,while a few were alkaline,and all NAC proteins were hydrophilic.Sequence analysis revealed that the 58 NAC family members in honeysuckle contained 10 motifs,with most family members harboring motifs 1,2,3,4,5,6,7,and 10.Among them,motif 5 and motif 3 were mostly connected.Lj4A633T23 exhibited the highest expression levels in both white and yellow honeysuckle flowers,with higher expression in white flowers than in yellow ones,followed by Lj1A850G59,which showed significantly lower expression in white flowers compared to yellow ones.GO functional annotation indicated that the 58 NAC transcription factors participated in 8 biological processes,2 molecular functions,and 3 cellular components.