月季花FRIGIDA基因家族的全基因组鉴定及生物信息学分析OA

Taking Rosa chinensis as the experimental material,bioinformatics methods included genome-wide identification combined with chromosomal localization,phylogenetic tree construction,synteny analysis,and promoter cis-acting element prediction were carried out to investigate the molecular characteristics and evolutionary mechanism of the RcFRI gene family,in order to provide reference for revealing the functional differentiation of this family in the growth,development and stress adaptation of Rosa chinensis.The results showed that 21 RcFRI members were identified distributed across 7 chromosomes in Rosa chinensis.The molecular weights of the proteins they encoded ranged from 54 to 111 kDa.18 RcFRI were localized in the nucleus.3 RcFRI showed nucleocytoplasmic dual localization.Phylogenetic tree analysis indicated that this family,along with homologous genes from Oryza sativa and Arabidopsis thaliana,was categorized into 5 subgroups.Subgroup V,which contained 16 RcFRI members,had Rosa chinensis enrichment,suggesting its participation in regulating species traits.Gene structure analysis verified that all members had UTR and CDS domains.Conserved motifs 2/3 were the most extensively distributed.The conserved domain belonged to the FRIGIDA superfamily.In the promoter region,a substantial number of light elements(Light),abscisic acid elements(ABA),and hormone elements were detected,meaning this family might mediate light signal transduction and stress responses.Synteny analysis disclosed 7 duplication events within the family.Only 3 pairs of homologous genes were recognized with Arabidopsis thaliana,showing a relatively remote evolutionary relationship.