裂果薯皂苷Ⅰ体外抑制鼻咽癌细胞HONE-1作用及其机制OA

Objective To explore the inhibitory effect and related molecular mechanisms of Saponin of Schizo-capsa plantaginea HanceⅠ(SSPHⅠ)on human nasopharyngeal carcinoma HONE-1 cells.Methods The effect of SSPHⅠ on HONE-1 cell viability was detected using the CCK-8 assay.Its inhibitory effect on cell proliferation was evaluated through a colony formation assay.Changes in cell invasion ability were analyzed using the Transwell assay.Intracellular reactive oxygen species(ROS)levels were measured using the DHE fluorescent probe.The ex-tent of intracellular content release was reflected by the LDH release assay.The rate of cell pyroptosis was detected using the Annexin-V/PI double staining method.Changes in the expression of proteins related to the classical py-roptosis pathway were examined by Western Blot.Results CCK-8 assay showed that treatment with SSPHⅠ for 24 hours reduced HONE-1 cell viability in a concentration-dependent manner,with an IC50 value of 3.383 μmol/L.In the colony formation assay,the number of HONE-1 cell colonies gradually decreased with increasing concentra-tions of SSPHⅠ(P<0.01).The Transwell assay revealed that the number of cells migrating through the chamber was reduced following SSPHⅠ treatment(P<0.01).DHE fluorescence probe detection indicated that intracellu-lar ROS fluorescence intensity increased after SSPHⅠ treatment(P<0.001).The LDH release assay showed that LDH activity in the cell supernatant increased with higher concentrations of SSPHⅠ(P<0.001).Annexin-V/PI double staining demonstrated that the proportion of Annexin-V/PI-positive cells increased after SSPHⅠ treatment(P<0.001).Western blot analysis showed that,compared with the control group,the protein expression levels of cleaved-Caspase-1 and GSDMD-N-terminal were upregulated in SSPH Ⅰ-treated cells(P<0.05),and NLRP3 protein expression levels also increased(P<0.05).ELISA results showed that the levels of IL-1β and IL-18 in the cells increased with higher concentrations of SSPHⅠ(P<0.05).Conclusion SSPHⅠ can induce pyroptosis in nasopharyngeal carcinoma HONE-1 cells by regulating the ROS/NLRP3/Caspase-1 signaling axis,thereby exert-ing an anti-nasopharyngeal carcinoma effect.This suggests that SSPHⅠ may serve as a potential therapeutic agent for nasopharyngeal carcinoma.