木犀草素防治聚苯乙烯微塑料诱导的血管损伤机制研究OA

Objective To explore the vascular endothelial injury in male mice caused by exposure to polystyrene microplastics(PS-MPs)and the intervention effect of luteolin on vascular remodeling.Additionally,to investigate the mechanism through the oxidative system and metabolomics.Methods Thirty-two C57BL/6 mice(6-8 weeks old)were randomly divided into the saline group(saline group),the 0.1 mg/kg PS-MPs exposure group(0.1PS-MPs group),the 1 mg/kg PS-MPs exposure group(1PS-MPs group),and the 1 mg/kg PS-MPs+luteolin treatment group(1PS-MPs+Lut group),with 8 mice in each group.After 8 weeks of intervention,the body weight,blood pressure,aortic organ coefficient,and aortic histopathological changes of mice in each group were detected;the to-tal cholesterol(TC),triglyceride(TG),and high-density lipoprotein cholesterol(HDL-C)lipid metabolism-related indicators in the aorta of mice were detected;the reactive oxygen species(ROS),glutathione(GSH),and malondialdehyde(MDA)oxidative stress-related indicators were detected;the endothelin(ET-1),nitric oxide(NO),vascular endothelial growth factor A(VEGF-A),vascular cell adhesion molecule-1(VCAM-1/CD106),and intercellular adhesion molecule-1(ICAM-1/CD54)endothelial function-related indicators and serum metabolo-mics were detected.Results Compared to the saline group,exposure to PS-MPs resulted in pathological thicken-ing of the mouse aorta,increased aortic organ coefficient,and elevated blood pressure.Lipid metabolism-related indicators,including TC and TG,were elevated,while HDL-C was reduced,indicating lipid metabolism disorder in mice.Oxidative stress markers such as ROS and MDA increased,whereas GSH decreased,demonstrating oxida-tive damage.Vascular endothelial inflammation and injury markers,including ET-1,VEGF-A,VCAM-1,and ICAM-1,were upregulated,while the vasodilatory substance NO was downregulated,confirming endothelial in-jury.Furthermore,serum metabolomics results revealed that PS-MPs exposure induced endothelial damage by dis-rupting metabolic pathways such as the citrate cycle.Compared to the PS-MPs group,luteolin significantly re-versed these effects,attenuating oxidative stress and lipid metabolism disorders,and effectively repairing endothe-lial injury.Conclusion PS-MPs induce vascular toxicity through oxidative stress and lipid metabolism.Luteolin effectively alleviates endothelial damage and vascular remodeling.