采用免疫磁珠捕获念珠菌释放的幽门螺杆菌的研究OA

Objective To investigate the ability of clinically isolated,Helicobacter pylori(H.pylori)-specific gene polymerase chain reaction(PCR)-positive gastric,vaginal,and fecal Candida to release H.pylori.Meth-ods Resuscitate 4 strains of H.pylori-specific 16S rDNA and ureA gene PCR-positive Candida strains isolated in laboratory from clinical sources,including 1 strain of gastric Candida,1 strain of fecal Candida,2 strains of vagi-nal Candida and the standard Candida albicans strain ATCC10231(Ca10231).The presence of H.pylori-specific ureA in the 5 strains of Candida isolates was confirmed by PCR.The aforementioned strains of Candida and H.py-lori were inoculated into urea medium and cultured in a constant temperature incubator at 37℃.The color change of the medium was observed daily.A change in the medium's color from yellow to red indicated the presence of ure-ase activity.Then,the five strains of Candida and H.pylori were co-incubated with the magnetic beads coated with H.pylori antibodies respectively.Scanning electron microscopy(SEM)was employed to observe the presence of bacilli adsorbed on the surface of the magnetic beads.PCR was used to detect the presence of H.pylori-specific 16S rDNA and ureA genes on magnetic beads.Results The PCR analysis of the ureA gene in the four Candida iso-lates was positive,whereas the Ca10231 strain tested negative.Upon culturing the four Candida isolates on urea medium,the medium color changed from yellow to red which was determined to be urease positive,while the me-dium containing Ca10231 remained unchanged,which was urease negative.SEM revealed that bacilli could be ob-served on the surface of magnetic beads co-incubated with the 4 strains of Candida of clinical origin and H.pylori isolate.Specifically,PCR testing of the magnetic beads co-incubated with one vaginal Candida,one gastric Can-dida and H.pylori isolate showed positive results for the 16S rDNA and ureA genes of H.pylori;however,the PCR tests for the two genes were negative for the magnetic beads co-incubated with the other two Candida isolate.Con-clusion This study demonstrates that H.pylori-specific genes Candida can release H.pylori.