Dietary β-hydroxybutyrate sodium alters rumen microbiome and nutrient metabolism in the rumen epithelium of young goatsOA
The role ofβ-hydroxybutyric acid(BHBA)includes providing energy,regulating signaling pathways,and ameliorating the gut microbiota in the host,while its nutrient mechanism to improve rumen epithelium development in young ruminants is still unclear.In this study,a total of 12 female Haimen goats with 30 d of age were chosen and divided into two groups.One group was fed with basic diet(CON),and the other group was fed a basal diet supplemented with 6 g d^(-1)dietaryβ-hydroxybutyrate sodium(BHBA-Na).The experimental period was 30 d,and all goats were slaughtered at 60 d of age.The joint analysis of multi-omics,including rumen microbiota,rumen epithelial transcriptome and rumen epithelial metabolomics in young goat model,was performed to systematically investigate the effect of dietary BHBA-Na on rumen development in young goats.As the results,we found that dietary BHBA-Na improved the growth performance of young goat including body weight,average daily gain(ADG)and dry matter intake(DMI)(P<0.05).Dietary BHBA-Na also increased the weight of rumen,and promoted the growth of rumen epithelium development(P<0.05).The abundance of several beneficial bacteria was increased(Fibrobacter,Succinivibrio,Clostridiales,etc.).The rumen epithelium transcriptome and metabolomics indicated that BHBA-Na supplementation showed a remarkable effect on the nutrient metabolism of the rumen epithelium.Specifically,the pathways of“fatty acid metabolism”,“cholesterol homeostasis”,“reactive oxygen species(ROS)pathway”and“peroxisome”were activated in response to BHBA-Na addition(P<0.05).Moreover,the genes(HMGCS2,ECSH1,ACAA2,ECH1,ACADS etc.)and metabolites(succinic acid,alpha-ketoisovaleric acid,etc.)involved in these pathways were also regulated positively(P<0.05).The rumen epithelium obtained the energy for its development from the process of volatile fatty acids(VFAs)decomposition.Finally,we observed the close correlations among the phenotypes,ruminal microbiota,host genes and epithelial metabolites.Overall,our results revealed that the BHBA-Na promoted the growth and rumen development of young goats possibly by enhancing DMI and regulating the rumen microbiota and the metabolisms of VFA and amino acid in the rumen epithelium.
Yimin Zhuang;Guanglei Liu;Chuyun Jiang;Mahmoud M Abdelsattar;Yuze Fu;Ying Li;Naifeng Zhang;Jianmin Chai
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,School of Animal Science and Technology,Foshan University,Foshan 528000,China Key laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,ChinaState Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China Jockey Club College of Veterinary Medicine and Life Sciences,City University of Hong Kong,Hong Kong SAR 999077,ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,School of Animal Science and Technology,Foshan University,Foshan 528000,ChinaKey laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China Department of Animal and Poultry Production,Faculty of Agriculture,South Valley University,Qena 83523,EgyptKey laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,School of Animal Science and Technology,Foshan University,Foshan 528000,ChinaKey laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,School of Animal Science and Technology,Foshan University,Foshan 528000,China Department of Animal Science,Division of Agriculture,University of Arkansas,Fayetteville AR 72701,USA
农业科技
goatsβ-hydroxybutyrate acidrumen microbiotarumen transcriptomerumen epithelial metabolomicrumen epithelium development
《Journal of Integrative Agriculture》 2026 (4)
P.1619-1635,17
funded by the Guangdong Provincial Modern Agricultural Industrial Technology System Innovation Team Construction Project(2024CXTD13)the Key Research and Development Plan Project of the Tibet Autonomous Region(XZ202301ZY0008N)funded by the Regional Science and Technology Collaborative Innovation Project of the Tibet Autonomous Region(SNQYKJXT-03 and QYXTZX-RKZ2024-03-1)。
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