Identification of broad-spectrum B-cell and T-cell epitopes of H9 subtype avian influenza virus HA protein using polypeptide scanningOA
The H9N2 subtype avian influenza virus(AIV)hemagglutinin(HA)protein is a major immunogen in which HA1 is a genetic variant and HA2 is relatively conserved.Identifying broad-spectrum antigen epitopes targeting HA1 is crucial for vaccine design and detection.Based on the phylogenetic and serological analyses,we identified 2 antigenic groups and 3 representative viruses:A/chicken/Jiangsu/JY040218C/2019,A/pigeon/Jiangsu/JY020616/2019,and A/chicken/Jiangsu/WX090312/2018.An overlapping peptide library was synthesized using HA1 amino acid sequences of the viruses as templates.Through peptide scanning of the sera against different strains of H9N2 subtype AIV,we identified peptides from 4 regions(H9-2/3,H9-20/21,H9-26,and H9-29/30/31)that demonstrated broad-spectrum reactivity.Immunological assay results demonstrated that H9-21(^(219)RIFKPLIGPRPLVNGLMGRI^(239)),H9-26(^(269)SGESHGRILKTDLKMGSCTV^(289)),and H9-30(^(309)YAFGNCPKYI GVKSLKLAVG^(329))effectively induced antibody generation and conferred partial protective efficacy against the parent virus JY040218C.The results of lymphocyte proliferation and ELISpot assays indicated that peptides H9-15(^(159)MRWLTQKNNAYPTQDAQYTN^(179)),H9-22(^(229)PLVNGLMGRINYYWSVLKP G^(249)),and H9-23(^(239)NYYWSVLKPGQTLRIKSDGN^(259))could effectively stimulate the expression of interferon-gamma in peripheral blood lymphocytes of chickens immunized against different strains of H9N2 AIV.Collectively,5 novel cell epitopes H9-15,H9-22,H9-23,H9-26,and H9-30,including the best B cell epitope H9-26 and the best T cells epitope H9-22,were identified that could be targeted for vaccine design or detection approaches against H9N2 AIVs.
Keji Quan;Nan Zhang;Mengqi Lin;Yuan Liu;Yue Li;Qun Hu;Maoshun Nie;Tao Qin;Jingzhi Li;Hongwei Ma;Sujuan Chen;Daxin Peng;Xiufan Liu
College of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,China Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education,Yangzhou 225009,China Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease,Yangzhou 225009,ChinaDivision of Nanobiomedicine,Suzhou Institute of Nano-Tech and Nano-Bionics,Chinese Academy of Sciences,Suzhou 215000,ChinaDivision of Nanobiomedicine,Suzhou Institute of Nano-Tech and Nano-Bionics,Chinese Academy of Sciences,Suzhou 215000,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,China Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education,Yangzhou 225009,China Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,China Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education,Yangzhou 225009,China Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease,Yangzhou 225009,ChinaCollege of Veterinary Medicine,Yangzhou University/Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,China Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education,Yangzhou 225009,China Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease,Yangzhou 225009,China
农业科技
H9N2 subtype avian influenza virusHA proteinepitopemicroarraypeptide
《Journal of Integrative Agriculture》 2026 (4)
P.1636-1646,11
supported by the National Key Research and Development Program of China(2021YFD1800202)the National Natural Science Foundation of China(3237042 and 32172942)the“Jie Bang Gua Shuai”Project at Yangzhou University,China(YZUXK202316)the Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province,China(SCX[22]3547)the Outstanding Technological Innovation Team of College and University at Jiangsu Province,China([2021]NO.1)a project funded by the Priority Academic Program Development of Jiangsu Higher Education,China(PAPD)。
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