A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assayOA
Highlights·By integrating RT-RAA with CRISPR/Cas12a,a portable,one-pot visual detection system was engineered,significantly minimizing the potential for false positives caused by aerosol contamination.·RT-RAA-CRISPR/Cas12a assay can complete the detection within 40 min,with a sensitivity as high as 3.9 copiesμL^(-1)for single copy.Porcine deltacoronavirus(PDCo V)disease is an acute and highly contagious illness caused by the PDCo V.
Zezhao Cao;Zi Li;Kaikai Jin;Zanheng Huang;Ruijie Hu;Junchao Shi;Huabo Yu;Gaili Wang;Wenqi He;Hualei Wang;Yungang Lan;Haili Zhang
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaJilin Academy of Animal Husbandry and Veterinary Medicine,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,China
农业科技
CRISPR Casaerosol contamination rt raa crispr caspdco vPorcine deltacoronavirusVisual detectionFalse positivesRapid detectionRT RAA
《Journal of Integrative Agriculture》 2026 (4)
P.1736-1739,4
financially supported by the National Key Research and Development Program of China(2021YFF0703600)。
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