Establishment and evaluation of a stable CHO cell line in which the nanobody PD-L1-Fc gene is precisely targeted into the C12orf35 locusOA
Chinese hamster ovary(CHO)cells are an extensively used platform for manufacturing biopharmaceuticals,and nearly 80%of recombinant protein is produced by CHO cell lines[1].Randomly incorporating genes of interest into the genome is a common method for the development of stable CHO cell lines in industry,but it is vulnerable to genetic instability,is difficult to predict productivity,and is accompanied by a time-consuming and laborious screening process,[2].Nonetheless,highly productive clones isolated from a randomized pool often exhibit unfavorable properties including transgene copy number loss and epigenetic silencing over the lifespan of the culture,ultimately lowering transgene transcription and corresponding recombinant protein production,which referred to as production instability[3,4].
Feng Chang;Chen Zhang;Yu Feng;Wenyun Zheng;Xingyuan Ma
State Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai 200237,ChinaState Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai 200237,ChinaShanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,ChinaShanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,ChinaState Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China
生物科学
genetic instabilityisincorporating genes interest genomeevaluationmanufacturing biopharmaceuticalsandrecombinant proteincho cell linesscreening process nonethelesshighlyestablishment
《Acta Biochimica et Biophysica Sinica》 2026 (2)
P.453-457,5
supported by the grants from National Key Research and Development Project of China (No. 2018YFA0902804),the National Natural Science Foundation of China (Nos. 32471003).
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