复合植物精油纳米乳稳定性、安全性及消毒效果的研究OA

This study was conducted to assess the stability,safety,and disinfection efficacy of the compound plant essential oil nanoemulsion disinfectant.The stability profile was determined through a series of stress tests,including centrifugation,high-temperature storage,freeze-thaw cycling,and dilution in water.Safety was meticulously evaluated using established protocols for skin irritation,ocular irritation,and in vitro cytotoxicity.The disinfectant's efficacy was quantified via the quantitative suspension test,object surface disinfection test,air disinfection test,and hand disinfection test.The results demonstrated that the compound plant essential oil nanoemulsion exhibits excellent physical stability.Following all stress treatments—centrifugation,high temperature,freeze-thaw cycles,and dilution—the formulation maintained a light yellow hue,remained clear and transparent,and showed no evidence of instability,such as phase separation,demulsification,or precipitation.The safety assessments classified the product as non-irritating to the skin and eyes,and it exhibited low cytotoxicity,confirming a favorable safety profile.In terms of efficacy,exposure to the disinfectant at its full concentration and at 2-fold and 4-fold dilutions for durations of 1 minute and 3 minutes and at 6-fold dilutions for durations of 3 minutes consistently yielded an average logarithmic reduction value of≥5 against Salmonella enteritidis.The product achieved an average logarithmic reduction value of 7.91 against natural flora on inanimate surfaces,a 100%bacterial killing rate during air disinfection,and an average logarithmic reduction value of 2.59 against natural microflora on the surface of the hands.The compound plant essential oil nanoemulsion demonstrates high stability and safety,along with satisfactory disinfection efficacy,making it suitable for the disinfection of inanimate surfaces,air,and dermal applications(hands).