基于大孔吸附树脂动态吸附解吸原理探究炒决明子中α-葡萄糖苷酶抑制剂OA

Objective:This study aims to investigate the potential active components responsible for α-glucosidase inhibition in fried CASSIAE SEMEN using dynamic correlation analysis.Methods:Differences in the adsorption and de-sorption rates of different compounds on macroporous adsorption resin were utilized to perform gradient elution of fried CASSIAE SEMEN extract with ethanol at different concentrations.The chemical components amount matrix[X]was es-tablished by analyzing the peak areas of compounds in the eluates using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS).The inhibition of α-gluco-sidase by eluates of different ethanol concentrations was measured to establish the bioactivity matrix[Y].Partial least squares(PLS)regression analysis was then applied to correlate matrix[X]and[Y],predict the effective components with α-glucosidase inhibitory activity in fried CASSIAE SEMEN,and verify the activity of selected compounds.Results:Reliable PLS models were established(positive ion mode:R2X=0.753,R2Y=0.998,Q2=0.982,negative ion mode:R2X=0.833,R2Y=0.999,Q2=0.993).A total of 86 and 64 active compounds were predicted in the positive and negative ion modes,respectively,with 31 active components ultimately identified.These components were mainly anthraquinones,flavonoids,and naphthopyranones.The activities of quercetin and kaempferol were verified with IC50 values of 4.5 μg/mL and 7.6 μg/mL,respectively.Conclusion:The dynamic adsorption-desorption method using macroporous adsorption resin effectively addressed the multicollinearity issues inherent in static correlation analysis and provided a no-vel approach for discovering active components in complex systems.The results confirm that fried CASSIAE SEMEN pos-sess α-glucosidase inhibitory activity,primarily attributed to anthraquinones,flavonoids,and naphthopyranones.