疏胃方配方药液对肝郁脾虚型慢性萎缩性胃炎大鼠胃黏膜菌群多样性的影响OA

Objective:To investigate the structural and abundance changes of the gastric mucosal microbiota in rats with chronic atrophic gastritis(CAG)of liver depression and spleen deficiency type,as well as to observe morphological alterations in the gastric mucosa via electron microscopy.By examining changes in the gastric mucosal microbiota structure,this study explores the effects of long-term administration of the Shuweifang formula liquid(疏胃方配方药液)on the abundance of gastric microbiota and its impact on gastric mucosal morphology in rats.Methods:Fifty specific pathogen-free(SPF)male SD rats,after passing quarantine,were selected,with 10 randomly assigned as the normal control group and the remaining rats designated as the model group.A multifactorial modeling method primarily using MNNG was optimized to establish a rat model of CAG with liver depression and spleen deficiency.The experimental rats meeting weight criteria were then randomly divided into three groups:the model control group,the Weifuchun(胃复春)group,and the Shuweifang formula liguid group,with 10 rats in each group.The normal control and model control groups were administered an equal volume of purified water via gavage,while the other treatment groups received the Shuweifang formula liquid at a dose of 0.9 mL/100 g once daily for 8 weeks.After the experiment,gastric mucosal tissues were collected from the antrum of the rats,and pathological sections were prepared with HE staining.The gastric mucosal lesions in each group were observed under an electron microscope.Gastric mucosal tissue samples were extracted,and the bacterial 16S rDNA-V3-V4 region was sequenced using the Illumina HiSeq PE250 high-throughput sequencing system.This process enabled quantitative analysis of the structure and abundance of the gastric mucosal microbiota.Results:The Shuweifang formula liquid group showed a significant decrease in the Chao1 and Observed species indices.At the phylum level,the abundance of Actinobacteriota,Bacteroidota,and Campylobacterota in the Shuweifang formula liquid group was significantly reduced(P≤0.05).Additionally the abundance of Lactobacillus was significantly increased(P≤0.05),the abundance of Monoglobus and Christensenella was significantly decreased(P≤0.05).Conclusion:The Shuweifang formula liquid may improve gastric inflammation by modulating the abundance of the gastric mucosal microbiota,thereby exerting a therapeutic effect on CAG.