基于"病-症-方-药"关联分析吴茱萸的妇科用药规律研究OA

Objective:Systematically summarize the prescribing regularity of Wuzhuyu(Fructus Evodiae)in gynecology of traditional Chinese medicine(TCM)to provide data support and direction reference for rational drug use and new drug research.Methods:Using"Wuzhuyu""gynecology",and specific diseases and syndromes as keywords,databases including the Dictionary of Chinese Medical Formulas,Traditional Chinese Medicine Classic Prescriptions database,Traditonal Chinese Medicine Heritage Family,Traditional Chinese Medicine Resource Network,CNKI,Wanfang Medical Network were searched.According to the inclusion and exclusion criteria,the relevant prescription data information was integrated,and Microsoft Excel 2019 was used to establish the database after standardization.Statistical analysis tools such as SPSS Modeler 18.0 and SPSS Statistics 22.0 were used to conduct the association analysis of"disease-syndrome-prescription-medicine",and the analysis results were mined and visualized from the perspectives of main disease syndrome,dosage,usage,processing and compatibility.Active ingredients and targets of Wuzhuyu were obtained in TCMSP,Swiss database,and PharmMapper database.Diseases targets were obtained from GeneCards database,and overlapping targets were screened out.Cytoscape 3.9.0 software was used to map the Wuzhuyu-diseases-target protein network.GO enrichment analysis and KEGG pathway analysis were performed on intersection targets by DAVID.Intersection targets were imported into the STRING database to obtain a protein-protein interaction network diagram,and Cytoscape 3.9.0 was used for visualization and screening of core targets.Results:A total of 296 relevant prescriptions were included,involving 53 kinds of diseases and syndromes.Among them,the high-frequency compatible herbs were mainly Danggui(Radix Angelicae Sinensis),Guizhi(Ramulus Cinnamomi),and Chuanxiong(Rhizoma Ligustici Chuanxiong).The main types of herbs were stasis-removing and blood-activating herbs.The main flavors were pungent,sweet and bitter and the main natures were warm and neutral.According to meridian tropism,the herbs mainly entered the spleen,heart,kidney meridians.The high-frequency major syndromes were low back pain and periumbilical pain,menstrual disorder,and most of them occurred during the menstrual period.The common dose was usually below 100 g,which mostly exceeded the dosage prescribed by Chinese pharmacopoeia."Wuzhuyu-Guizhi-Danggui"were the core compatibility combination.The key signaling pathways obtained from enrichment analysis mainly included the PI3K-Akt signaling pathway,AGE-RAGE signaling pathway,and endocrine resistance signaling pathway,among others.The key targets obtained from PPI network analysis included AKT1,STAT3,ESR1,among others.Conclusion:The compatibility pairs of Wuzhuyu were mainly composed of tonic herbs,which were primarily indicated for TCM gynecological disorders such as irregular menstruation and periumbilical pain.The therapeutic method was dominated by tonification,while the administration methods and processing approaches were designed with the primary goal of reducing toxicity.There may be a synergistic and toxicity-reducing effect in the compatibility of Wuzhuyu with Danggui,Renshen(Radix Ginseng),Ganjiang(Rhizoma Zingiberis)and other herbs.This study may provide evidence for the safe and rational clinical application of Wuzhuyu in gynecology,and may offer valuable insights into promoting the upgrade of its pharmacopoeia quality standards as well as the research on herb compatibility and prescriptions based on efficacy-toxicity correlation evaluation.