基于网络药理学探讨十枣汤干预心力衰竭的作用机制OA

This study investigates the mechanism of action of the traditional Chinese medicine formula Shizao decoction(十 枣 汤)in heart failure.Employing network pharmacology methods for systematic analysis,the research integrates multiple data platforms and bioinformatics tools to construct a multi-layered interaction network between the compound formula and complex diseases.Firstly,by means of the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),oral bioavailability and drug-likeness were set as screening criteria to identify 61 candidate molecules with potential activity,and the corresponding 237 target genes were obtained.Subsequently,Heart Failure(HF)-related targets were retrieved from GeneCards,the Online Mendelian Inheritance in Man(OMIM)database,and the Therapeutic Targets Database(TTD).After relevance score-based screening and deduplication,614 highly relevant targets were identified.Intersection analysis further pinpointed 50 overlapping targets which were imported into the STRING database to construct a protein-protein interaction network.Using Cytoscape topological parameters,core network nodes were identified,including key targets such as IL-1B,IL-6,and TNF,which play pivotal roles in regulating heart failure progression.During the construction of the"herbal medicine-active compound-target interaction"network,components such as quercetin,kaempferol,and β-sitosterol exhibited high network connectivity.This suggests these substances may be the primary bioactive constituents of the formula.GO analysis of core targets revealed widespread involvement in transcription regulation,cytokine effects,and immune processes.KEGG pathway enrichment analysis suggested potential mechanisms involving AGE-RAGE signaling,atherosclerosis,and inflammatory regulation.At the mechanistic level,quercetin may regulate apoptosis-related protein expression by modulating the MAPK signaling pathways,meanwile β-sitosterol may exert myocardial protection by alleviating oxidative stress and inflammatory responses,while kaempferol may enhance intracellular antioxidant capacity and optimize myocardial microenvironments.Overall,Shizao Tang demonstrates a comprehensive intervention advantage characterized by"multiple-target,multiple-component,multiple-pathway",which provides a theoretical basis for exploring its mechanisms in heart failure intervention and facilitating its translation into modern clinical application.