茄子嫁接后愈合过程中组织结构和生理特性变化研究OA

This study used wild Solanum torvum Swartz.as rootstock and cultivated eggplant Solanum melongena L.Yunqie 9 as scion.The grafting method was cleft grafting.The structure of graft union at 0,2,4,6,8,10,and 12 days after grafting was observed,and physiological indicators such as phenylalanine ammonia lyase(PAL),peroxidase(POD),superoxide dismutase(SOD),and soluble protein(SP)were measured to study the tissue structure and physiological characteristics during healing process after eggplant grafting.The results showed that the healing process of grafting between S.torvum Swartz.and Yunqie 9 could be divided into four stages,including isolation layer and callus formation stage(0-4 d),callus division and proliferation connection stage(5-7 d),cambium connection stage(8-9 d),vascular tissue differentiation and formation stage(10-12 d).The physiological indicators measured within 12 days after grafting showed that the changes in physiological characteristics of grafted seedlings and self-grafted seedlings were basically consistent.Among them,PAL activity showed an overall downward trend,SOD activity showed a"V"shaped change of first decreasing and then increasing,while POD activity and SP content showed an"M"shaped change.In addition,there were significant changes in most physiological indicators at four days after grafting;After six days,the SOD activity of all treatments showed an overall increase,and the increasing trend of SOD activity in grafted seedlings was more pronounced,with a difference of 19 times between the highest and lowest values;During the 6th to 8th days,the SP content of grafted seedlings rapidly increased and reached its highest value on the 8th day,which was 5.6 times the lowest value.In summary,the healing process between S.torvum Swartz.and Yunqie 9 could be basically completed 12 days after grafting,and the key healing time points might be 4,6,and 8 days after grafting.