73份甘肃陇南冬小麦品种(系)抗条锈病基因分子检测OA

To investigate the resistance levels to currently prevalent races and new isolates,as well as the distribution of stripe rust resistance genes in released and candidate wheat varieties from Longnan,Gansu,73 wheat varieties(lines)were evaluated for seedling resistance against the prevalent races CYR32,CYR34,and ZS asexual isolate and ZS sexual isolate.Meanwhile,molecular markers linked to all-stage resistance genes Yr5,Yr9,Yr10,Yr15,Yr26,and the adult-plant resistance gene Yr18 were utilized for molecular detection.The results indicated that among the 73 tested wheat varieties(lines),11(15.07%),11(15.07%),13(17.81%),and nine(12.33%)exhibited seedling resistance to CYR32,CYR34,and the asexual and sexual isolates of ZS,respectively.Among all tested materials,only Zhongliang 14 exhibited seedling resistance to all the provided isolates of stripe rust.Molecular detection results revealed that the number of wheat varieties(lines)carrying Yr5,Yr9,Yr10,Yr18,and Yr26 was 1,27,3,3,and 16,respectively,accounting for 1.37%,36.99%,4.11%,4.11%,and 21.92%of the total tested materials.Three materials carried two resistance genes,and Zhongliang 14 carried three resistance genes.The Yr15 gene was not detected in any of the tested varieties(lines),and 28 materials carried none of the six tested resistance genes.