马铃薯StMYB3基因的克隆、生物信息学及其表达分析OA

The influence of potato transcription factors on anthocyanin accumulation is primarily reflected in their regulation of genes involved in the anthocyanin biosynthetic pathway.In this study,the StMYB3 gene was cloned by RT-PCR from three potato cultivars:Shepody(white flesh),Wanziqianhong(light purple flesh),and 2013-89-61(dark purple flesh).Bioinformatics characterization and differential expression analysis were performed.The results showed that the target fragment of StMYB3 genes was 710 bp in length,encoding a protein with a molecular weight of 20.71 kDa and an isoelectric point of 9.14,which was identified as a hydrophilic protein.StMYB3 genes contains R2R3-MYB domain and bHLH interaction motif but lacks a transmembrane domain.Phylogenetic analysis revealed that StMYB3 genes shares the highest sequence similarity with the ScMYB gene from pepper.Semi-quantitative PCR results showed that the expression level of potato StMYB3 gene in different tissues is:tender leaf>old leaf>stem>tuber>root,and the cultivar 2013-89-61 exhibited consistently high expression across all tissues.