黄淮南片麦区新育成小麦品种(系)高分子量麦谷蛋白遗传多样性分析OA

To clarify the relationship between high-molecular-weight glutenin subunits(HMW-GS)composition and quality traits in newly developed wheat varieties(lines)in the Southern Huang-Huai Wheat Region,a total of 93 newly developed wheat varieties(lines)from this region were used as materials.The HMW-GS composition was identified using SDS-PAGE,and their distribution and quality characteristics were systematically analyzed in conjunction with grain quality traits.The results showed that nine subunit types were detected across the three Glu-1 loci,specifically,subunits 1(72.04％)and Null(27.96％)at the Glu-A1 locus;subunits 7+9(64.52％),7+8(30.11％),and 14+15(5.38％)at the Glu-B1 locus;and subunits 2+12(37.63％),5+12(32.26％),5+10(29.03％),and 2+10(1.08％)at the Glu-D1 locus.Among the 15 identified subunit combinations,the most frequent combination was 1/7+9/2+12(19.35％),while the frequency of the combination 1/7+8/5+10 was 8.60％.Based on quality traits,the tested materials were clustered into three groups,with Group Ⅰ,Group Ⅱ,and Group Ⅲ accounting for 47.3％,17.2％,and 35.5％,respectively.Group Ⅲ exhibited the best performance across all quality indicators such as protein content,water absorption rate,and wet gluten content;the frequencies of subunits 14+15,7+8,and 5+10 in group Ⅲ were 6.06％,36.36％,and 30.30％,respectively.Varieties(lines)carrying the pedigree of two founder parents,Yumai 2 and Zhou 8425B,accounted for 70.96％of the tested materials.Eight varieties such as Zhengmai 366,Xinmai 26,Jimai 44,and Fumai 916,were screened for carrying high-quality subunits and combinations.These results provide a theoretical basis for parent selection in wheat quality breeding in the Southern Huang-Huai Wheat Region and serve as an important reference for further optimizing HMW-GS composition and improving wheat quality.