马铃薯对疮痂病致病毒素应答的差异蛋白质组学研究OA

To elucidate the proteomic response mechanism of potato to a pathogenic toxin Thaxtomin A(TA),causing common scab,this study was conducted using the potato cultivar Kexin 19 as the experimental material.Tandem Mass Tag(TMT)-based quantitative proteomics technology was employed to analyze the differential proteomes of potato leaves treated with the toxin and a control group.A total of 130 differentially expressed proteins(DEPs)were identified,including 80 up-regulated and 50 down-regulated.GO and KEGG enrichment analysis showed that the DEPs were significantly enriched in biological processes such as defense response,plant MAPK signaling pathway,S-adenosylmethionine biosynthesis,and protein processing in the endoplasmic reticulum.This reveals that the response of potato to the scab toxin involves a complex physiological and biochemical regulatory network.Through protein-protein interaction network analysis,the core protein M1ASG7 with the highest connectivity was screened out.Additionally,two up-regulated DEPs,M02PK2 and M1A0Y3,were found in its metabolic pathway,all of which are closely related to the regulation of cellular metabolism.