基于水培技术的甘薯种质弱苗复壮方法研究OA

The secure preservation of germplasm resources serves as a critical foundation for crop breeding,and developing efficient conservation methods for weak-growth accessions is particularly crucial.This study utilized 135 accessions of weak sweet potato seedlings as experimental materials.These materials were hydroponically cultured for 21 d to induce rooting,subsequently transplanted into potting substrate for continued conservation,with their post-transplant growth recovery systematically monitored.The results demonstrated that hydroponic treatment induced robust rooting in weak seedlings,with 78.5％of accessions(line)producing 4-18 roots per plant and 88.1％exhibiting an average root length of 0.6-3.1 cm.At 20 d post-transplantation,the overall survival rate recorded 82.2％.After five months,96％of the accessions(line)produced vine lengths longer than 30 cm,A significant increase was observed in basal branching number,while 91％attained stem diameters greater than 3.0 mm,confirming that the overall growth met the established criteria for successful rejuvenation.Virus elimination was subsequently applied to the hydroponically-rejuvenated accessions(line),achieving a success rate of 90.1％.Virus elimination significantly restored the tuberization capacity in conventional seedlings of non-tuberizing accessions.The results showed that 82.4％of accessions(line)with virus-free seedlings achieved a yield exceeding 1000 kg per mu,whereas only 27.5％of conventional seedlings from the same accession reached equivalent production levels.The hydroponic rejuvenation technique not only enhanced the survival capacity of weak germplasm seedlings but also secured a critical time window for subsequent virus elimination,thereby providing a reliable and viable approach for the rejuvenation and conservation of weak seedlings within sweet potato germplasm resources.