不同叶形五指毛桃补骨脂素和佛手柑内酯含量及转录特征比较OA
Comparative Analysis of Psoralen and Bergapten Contents and Transcriptome Characterization in Ficus hirta with Different Leaf Shapes
五指毛桃(Ficus hirta)是一种重要的药食两用植物,但叶片形态变异较大.为揭示其叶形差异与主要活性物质含量的关系,采用LC-MS/MS测定全缘型(EL)、浅裂型(LL)和五指深裂型(PDL)3种叶形五指毛桃根中补骨脂素和佛手柑内酯的含量,进一步利用转录组测序技术探讨其含量差异的分子机制.结果表明,PDL五指毛桃的补骨脂素含量显著高于EL和LL;EL五指毛桃的佛手柑内酯含量显著高于LL,但与PDL无显著差异.转录组测序共获得60.9 Gb clean reads,组装获得46 194个unigenes,同源序列比对显示五指毛桃与川桑(Morus notabilis)的同源性最高.从PDL_vs_EL、PDL_vs_LL和LL_vs_EL比较组分别筛选出2 355、2 067和2 001个差异表达基因(DEGs),DEGs主要富集在苯丙烷生物合成、淀粉和蔗糖代谢以及植物-病原体相互作用等通路上,其中苯丙烷生物合成和植物-病原体相互作用可能是五指毛桃主要活性物质生物合成与积累的重要通路.研究初步探讨了五指毛桃不同叶形与主要活性物质积累之间的关系,丰富了五指毛桃转录组数据库,为进一步利用不同叶形五指毛桃种质资源及育种提供参考依据.
INTRODUCTION:Ficus hirta is a common Chinese herbal medicine and edible plant resource in the Lingnan area of China.It contains coumarins,flavonoids and other chemical compounds,which have antioxidant,anti-inflammatory,antibacterial,antiviral and antitumor effects.However,F.hirta exhibits significant morphological variation in leaf shapes due to its high genetic diversity. RATIONALE:To investigate the relationship between leaf shape differences and the contents of major active com-pounds,LC-MS/MS was performed to determine the contents of psoralen and bergapten in F.hirta roots from three different leaf shapes(entire leaf(EL),lobed leaf(LL),and palmately deeply lobed leaf(PDL)),and transcriptome se-quencing was further employed to explore the potential molecular mechanisms underlying these content variations. RESULTS:The results showed that PDL exhibited significantly higher psoralen content than EL and LL,while EL had significantly higher bergapten content than LL,but no significant difference was observed between EL and PDL.A total of 60.9 Gb clean reads were obtained,and 46 194 unigenes were assembled.F.hirta had the highest homology with Morus notabilis,according to the homologous sequencing alignment.A total of 2 355,2 067 and 2 001 differentially expressed genes(DEGs)were screened from three comparison groups,PDL_vs_EL,PDL_vs_LL,and LL_vs_EL,re-spectively.These DEGs were primarily enriched in pathways such as phenylpropanoid biosynthesis,starch and sucrose metabolism,and plant-pathogen interactions. CONCLUSION:There were differences in the contents of psoralen and bergapten among the three leaf shapes of F.hirta.The phenylpropanoid biosynthesis and plant-pathogen interaction pathways may play crucial roles in the biosyn-thesis and accumulation of psoralen and bergapten in F.hirta.This study preliminarily revealed the correlation between the leaf morphology and major active compounds of F.hirta and extended its public transcriptome database,which will provide a reference for further utilizing different leaf shapes of F.hirta germplasm resources and quality breeding.
唐美琼;梁春英;胡营;林杨;韦范;梁莹;李林轩;李刚
广西壮族自治区药用植物园,国家中医药传承创新中心,西南濒危药材资源开发国家工程研究中心,广西药用资源保护与遗传改良重点实验室,广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023广西大学农学院,南宁 530004广西壮族自治区药用植物园,国家中医药传承创新中心,西南濒危药材资源开发国家工程研究中心,广西药用资源保护与遗传改良重点实验室,广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023广西壮族自治区药用植物园,国家中医药传承创新中心,西南濒危药材资源开发国家工程研究中心,广西药用资源保护与遗传改良重点实验室,广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023广西壮族自治区药用植物园,国家中医药传承创新中心,西南濒危药材资源开发国家工程研究中心,广西药用资源保护与遗传改良重点实验室,广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023广西壮族自治区药用植物园,国家中医药传承创新中心,西南濒危药材资源开发国家工程研究中心,广西药用资源保护与遗传改良重点实验室,广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023广西壮族自治区药用植物园,国家中医药传承创新中心,西南濒危药材资源开发国家工程研究中心,广西药用资源保护与遗传改良重点实验室,广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023广西壮族自治区药用植物园,国家中医药传承创新中心,西南濒危药材资源开发国家工程研究中心,广西药用资源保护与遗传改良重点实验室,广西壮族自治区中药资源智慧创制工程研究中心,南宁 530023
五指毛桃叶形活性成分LC-MS/MS转录组
Ficus hirtaleaf shapeactive ingredientsLC-MS/MStranscriptome
《植物学报》 2026 (2)
223-237,15
广西重点研发计划(桂科No.AB24010004)、广西自然科学基金(No.2025GXNSFAA069027)、广西药用植物保育人才小高地自主研究项目(No.GXYYXGD202201)和广西药用植物园重点研发计划(桂药重No.202305)
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