植物再生分子工具开发研究进展OA

The totipotency and pluripotency of plant cells serve as the theoretical basis for plant regeneration,underpin-ning various techniques such as plant tissue culture,genetic transformation,cutting,and grafting.In recent years,ad-vances in understanding the molecular mechanisms of plant regeneration-particularly key transcription factors,hormonal signaling networks and epigenetic factors-have led to the development of a series of molecular tools capable of ma-nipulating cell fate,offering new strategies to enhance regenerative capacity.This review summarizes the molecular and chemical tools that play central roles in four key regenerative processes:shoot regeneration,root regeneration,somatic embryogenesis,and grafting.It also discusses practical considerations such as delivery methods for these tools,selection of culture systems,and strategies for efficacy evaluation.Furthermore,the review explores future directions,including the rational combination of genetic and chemical tools to develop novel delivery systems and overcome epigenetic barriers,thereby providing theoretical and practical guidance for designing efficient regeneration protocols tailored to different species and regeneration objectives.