基于网络药理学探讨桂枝茯苓丸治疗子宫肌瘤的作用机制OA

Objective Investigating the potential mechanism of Guizhi Fuling Pills against uterine fibroids(UF)via network pharmacology.Methods Using the TCMSP database and ADME parameters(OB≥30%,DL≥0.18),the active ingredients of Guizhi Fuling Pills were screened;Predict the target of action of its active ingredients through the Pharmmapper database.Retrieve disease targets related to UF from the GeneCards database.Using Venn diagram to identify the common targets of drug disease interactions,and constructing a"component target pathway"network through Cytoscape.Results As a result,76 active ingredients and 149 targets of Guizhi Fuling Pills were screened out;Obtained 937 targets for UF disease;There are 29 common targets of drug disease interaction.Protein-protein interaction network analysis identified core targets such as ALB,ESR1,CASP3,EGFR,etc.Gene Ontology enrichment analysis mainly involves biological processes such as sighal transduction and response to estradiol.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggests that its effects are closely related to estrogen signaling pathway,MAPK signaling pathway,cancer pathway,and other pathways.Conclusion This study preliminarily indicates that Guizhi Fuling Pills acts on multiple core targets through multiple active ingredients,regulating multiple pathways such as estrogen signaling and MAPK signaling,thereby exerting therapeutic effects on UF.This research providing a theoretical basis for further pharmacological research.