花生WOX基因家族的全基因组分析及不定芽再生候选基因的鉴定OA

The WUSCHEL-related homeobox(WOX)transcription factor family plays central roles in maintaining stem cell ho-meostasis and regulating organogenesis in plants.Genetic transformation of peanut(Arachis hypogaea L.),a globally important oilseed crop,remains severely limited by low regeneration efficiency.Here,we conducted a genome-wide identification and characterization of WOX genes in the peanut cultivar'Fuhuasheng'to pinpoint candidates potentially involved in organ regenera-tion and to provide a basis for overcoming regeneration-related constraints in peanut transformation.We identified 20 WOX genes and systematically analyzed their phylogenetic relationships,conserved domains,collinearity,and promoter features,and then profiled their expression across tissues and during adventitious shoot regeneration using transcriptome data.Phylogenetic analysis clustered the AhWOX genes into three clades(ancient,intermediate,and WUS).All AhWOX proteins contained a conserved ho-meodomain(HD),and several WUS-clade members also carried the WUS-box and EAR motifs.Collinearity analysis indicated that AhWOX7,AhWOX12,and AhWOX14 are orthologs of Arabidopsis thaliana WUS and WOX5 and of the soybean regenera-tion-related gene GmWOX18,respectively.However,these orthologs showed only low expression,whereas the ancient-clade genes AhWOX13 and AhWOX20 were consistently highly expressed across all stages of adventitious shoot regeneration.Promoter analysis revealed enrichment of cis-elements associated with meristem activity,and phylogenetic evidence suggested that Ah-WOX13 and AhWOX20 are orthologous to AtWOX14,a reported positive regulator of shoot regeneration.Collectively,our results highlight AhWOX13 and AhWOX20 as promising candidates for improving regeneration capacity in peanut,providing a frame-work to enhance peanut transformation and accelerate functional genomics and trait engineering in oilseed crops.