川西小黄菊茎叶三种黄酮成分含量测定及抗炎活性部位筛选OA

To further develop and utilize the non-medicinal parts(stems and leaves)of Tanacetum tatsienense(T.tatsienense),HPLC method was employed to quantify the contents of three active flavonoids in these parts.The results indicate that all three flavonoids are present in the stems and leaves,with concentrations ranked from highest to lowest as follows:Luteolin at 0.2650 mg/g,tricin at 0.1720 mg/g,and apigenin at 0.1373 mg/g.Additionally,to evaluate the anti-inflammatory activity of the stems and leaves of T.tatsienense,four extracts(80％ethanol,petroleum ether,ethyl acetate,and n-butanol)were prepared using a systematic solvent extraction method.The CCK-8 assay was utilized to assess the toxicity of these extracts on RAW 264.7 cells and to determine their safe dosage.An inflammation model was established by inducing RAW 264.7 cells with LPS.The Griess method was employed to evaluate the inhibitory effects of the extracts on cellular NO release,while q-PCR was used to measure the expression levels of related inflammatory factor mRNAs.The findings revealed that the n-butanol extract of the stems and leaves of T.tatsienense exhibited no cytotoxicity at a concentration of 50 μg/mL,with an NO inhibition rate of 82.59％.This extract demonstrated anti-inflammatory effects by reducing the mRNA expression levels of TNF-α,IL-1β,and iNOS.Overall,the research suggests that the non-medicinal parts(stems and leaves)of T.tatsienense contain active ingredients similar to those found in the medicinal parts(inflorescences),possess notable anti-inflammatory activity,and hold potential for further development.