L6细胞中稳定敲除AMPKα1对抗糖尿病药物发挥作用的影响OA

The consumption of glucose by muscle cells is a key step in determining the level of blood sugar in the body.Adenosine 5'-monophosphate-activated protein kinase(AMPK),a key pathway in diabetes adenosine monophosphate,promotes glucose uptake in muscle cells when activated by phosphorylation.In order to explore the key role of AMPKαsubunit in the treatment of diabetes,AMPKα1 protein in L6 rat skeletal muscle cells was knocked out by using regularly clustered interspaced short palindromic repeats(CRISPR/Cas9)to explore the effect of AMPKα1 subunit deletion in L6 cells on the treatment of diabetes.The results showed that the metformin-promoted phosphorylation of AMPKα subunit completely disappeared when AMPKα was deleted,and the effect of metformin on glucose uptake in normal cells showed a decreasing trend in knockout cells.It reveals the key position of AMPKα1 subunit in the action of diabetic drugs,and provides an important experimental model for precise targeted drug therapy and drug mechanism exploration.