VIGS介导的NtHD20基因沉默促进烟草腺毛发生OA
VIGS mediated NtHD20 gene silencing promotes tobacco glandular trichome pilogenesis
本研究以8306野生型株系(WT)、纯合编辑株系(KD)和过表达株系(OE)为材料,探究病毒诱导的基因沉默(virus-induced gene silencing,VIGS)技术沉默NtHD20基因后对各株系的腺毛表型、腺毛密度、腺毛分泌物含量及其关键合成基因表达量变化的影响.结果表明,沉默NtHD20基因后各株系烟草叶面腺毛密度均极显著(p＜0.01)增加,腺毛分泌物含量及其关键合成基因表达量均显著(p＜0.05)增加.利用VIGS技术沉默NtHD20基因能够促进烟草腺毛发育,并提升西柏三烯醇类物质含量,推测NtHD20基因可能对烟草腺毛发育起负调控作用.本研究为改善烟草腺毛性状提供新的方向.
This study used the wild-type strain 8306(WT),homozygous edited strain(KD),and overexpressed strain(OE)as materials to investigate the effects of virus-induced gene silencing(VIGS)targeting the NtHD20 gene on the glandular trichome phenotype,glandular trichome density,content of glandular trichome secretions,and expression levels of key biosynthesis genes of glandular trichome secretions in all tobacco lines.The results showed that silencing the NtHD20 gene resulted in an extremely significant(p＜0.01)increase in leaf glandular trichome density,a significant(p＜0.05)increase in content of glandular trichome secretions,and expression levels of key biosynthesis genes of glandular trichome secretions in all tobacco strains.Silencing NtHD20 with VIGS technology can promote the development of tobacco glandular trichome and increase the content of cembratrieneols substances.It is speculated that the NtHD20 gene may have a negative regulatory effect on the development of the tobacco glandular trichome,providing a new direction for improving the tobacco glandular trichome.
徐鸣锴;高鑫;王东;鲁宇童;李红涛;陈孟起;段卫东;申洪涛;徐世晓
河南农业大学烟草学院,河南郑州 450002河南中烟工业有限责任公司,河南郑州 450016河南中烟工业有限责任公司,河南郑州 450016河南中烟工业有限责任公司,河南郑州 450016河南中烟工业有限责任公司,河南郑州 450016河南中烟工业有限责任公司,河南郑州 450016河南中烟工业有限责任公司,河南郑州 450016河南中烟工业有限责任公司,河南郑州 450016河南农业大学烟草学院,河南郑州 450002
农业科技
烟草腺毛腺毛分泌物病毒诱导的基因沉默
tobacco glandular trichomeglandular trichome secretionvirus-induced gene silencing
《浙江农业科学》 2026 (4)
878-883,6
中国烟草总公司河南省公司创新项目(2024410000240027)河南中烟工业有限责任公司科技项目(2024410000340192)安徽省烟草专卖局(公司)科技项目(2024341800040184)贵州省烟草公司安顺市公司重点研发项目(2024520400240023)
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