斜纹夜蛾雄蛾对种间性信息素的嗅觉反应OA

To elucidate the olfactory mechanism underlying interspecific chemical communication in Spodoptera litura,this study used S.litura as the subject of investigation.Through field trapping experiments,we determined the modulatory effects of interspecific pheromones on the trapping of male moths by sexual pheromones.Using two-electrode voltage clamp techniques,we characterized the responses of pheromone receptors of S.litura to interspecific pheromones from both S.litura and other pest species in its habitat.Field experiments revealed that interspecific pheromones,specifically Z7-12:Ac and Z9-14:OH,strongly reduced the trapping efficacy of S.litura males,and the effects of intraspecific trace components varied regionally.Electrophysiological analysis revealed that SlituOR6 exhibited a strong current response to E11-14:Ac and a weaker current response to 16:Ac.SlituOR16,however,displayed the highest sensitivity to the S.exigua sex pheromone component Z9-14:OH,a moderate sensitivity to the Agrotis ipsilon sex pheromone component Z7-12:Ac,and a weak response to the S.litura sex pheromone trace component E11-14:Ac.These findings suggest that SlituOR16 plays a dual role in detecting both intraspecific and interspecific pheromone signals.Fluorescence in situ hybridization(FISH)further localized SlituOR16 to receptor neurons at the base of the antennae,suggesting that SlituOR16 may be involved in the recognition of sex pheromones.Collectively,this study provides evidence of SlituOR6 and SlituOR16 specific responses to interspecific sex pheromone signals,elucidating the molecular basis of interspecific chemical communication in S.litura.Additionally,it highlights the interplay between receptor specificity and environmental background odors in modulating these interactions and offers a theoretical foundation for pheromone-based monitoring and control.