姜荷花粉色苞片花青素形成的关键基因及呈色机制分析OA

Curcuma alismatifolia is a perennial rhizomatous herbaceous flower belonging to the genus Curcuma,with flower color improvement serving as a key breeding objective.In this study,the anthocyanidin components in the pink sterile bracts of C.alismatifolia'Chiang Mai Pink'were analyzed using high performance liquid chromatography.Four anthocyanidins were identified:malvidin,delphinidin,cyanidin and pelargonidin,with malvidin exhibiting the highest proportion.The contents of cyanidin and malvidin increased significantly with the opening process of the flower,while the content of pelargonidin reached its peak when the flower was semi-open.Based on full-length transcriptome sequencing data from the PacBio platform,full-length sequences of nine key genes(PAL1,C4H1,4CL1,CHS1,F3H1,F3´H1,F3´5´H1,ANS1,and 3GT1)involved in the anthocyanidin biosynthesis pathway were obtained.Tissue-specific expression verification was performed for F3´5´H1,F3H1,and ANS1,revealing that their expression levels in sterile bracts and single flowers were significantly higher than those in fertile bracts and transitional bracts.Correlation analysis showed that the contents of cyanidin,delphinidin,and malvidin were positively correlated to varying degrees with the expression levels of the aforementioned nine genes and DFR(GenBank accession number GU126388.1),whereas the changes in pelargonidin content exhibited inconsistent correlations.Based on the comprehensive analysis of anthocyanidin composition,dynamic changes,and spatiotemporal characteristics of gene expression,it is concluded that the anthocyanidin substances responsible for the pink coloration of sterile bracts in'Chiang Mai Pink'are cyanidin and pelargonidin from the red series,along with a certain amount of delphinidin from the blue-purple series and its derivative malvidin;among them,malvidin plays an important role in the coloration of the purple petals of the florets.This study provides a theoretical basis for elucidating the molecular mechanisms underlying coloration in C.alismatifolia and for the targeted improvement of flower color.