赤芍中1个新的萜类化合物及其体外抗凝血活性OA

Twenty-one compounds were isolated and identified from the 80%ethanol extract of Paeoniae Radix Rubra by various chromatographic techniques such as D101 macroporous resin,C18 reversed-phase silica gel,and gel chromatography,combined with modern spectroscopic techniques including infrared spectroscopy(IR),ultraviolet(UV),mass spectrometry(MS),and nuclear magnetic resonance(NMR).These compounds were 3-formyl-5-hydroxy-6-methyl-1H-indole-1-butyric acid(1),3-hydroxymethyl-5-methyl-2,3-dihydrobenzofuran(2),paeoniflorin(3),5-hydroxy-6-methyl-1H-indole-3-carbaldehyde(4),5-hydroxy-3S-hydroxymethyl-6-methyl-2,3-dihydrobenzofuran(5),paeoveitol C(6),galloylpaeoniflorin(7),benzoylpaeoniflorin(8),4-O-galloylpaeoniflorin(9),1-borneol 6-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside(10),paeonidanin(11),paeonidanin E(12),paeonidanin B(13),palbinone(14),23-hydroxybetulinic acid(15),hederagenin(16),N-methylhydroxylamine(17),(5Z,9Z)-17-methylnonadeca-5,9-dienoate(18),3,3',4'-tri-O-methylellagic acid(19),β-sitosterylglucoside-6'-octadecanoate(20),and trilinolein(21).Among them,compound 1 was a new compound,and compounds 10 and 17-21 were isolated from the Paeoniae genus for the first time.All the compounds were tested for their anticoagulant activity through three coagulation function indicators:activated partial thromboplastin time(APTT),thrombin time(TT),and prothrombin time(PT).Compounds 1 and 7 showed excellent anticoagulant activity,which was comparable to that of the positive control sodium heparin.Compounds 2,3,5,6,8-13,15,and 19 exhibited good anticoagulant activity,while compounds 4 and 21 demonstrated moderate anticoagulant activity.