基于免疫胶体金的中药材外源污染多毒素同步快检技术研究OA

Mycotoxin contamination is one of the major causes of quality and safety risks in Chinese medicinal materials.To effectively ensure medication safety,this study established a multiplex and rapid detection method for exogenous fungal mycotoxins in Chinese medicinal materials.Flower-like gold nanoparticles(AuNPs)and gold-labeled probes were characterized using ultraviolet-visible spectroscopy and transmission electron microscopy.Key parameters,including the pH of the AuNP solution,antibody dosage,amount of gold-labeled probes,antigen-antibody jetting rate,nitrocellulose membrane selection,and the mixing ratios of multiple gold-labeled antibodies,were optimized.Gold-labeled probes capable of simultaneously detecting aflatoxin B1(AFB1),zearalenone(ZEN),ochratoxin A(OTA),deoxynivalenol(DON),and T-2 toxin(T2)were successfully prepared,with particle sizes of 90.16,85.81,83.12,88.10,and 87.54 nm,respectively.The limit of detection(LOD)for AFB1,ZEN,OTA,DON,and T2 were 2.5,5,2.5,5,and 2.5 ng·mL-1,respectively,meeting the mycotoxin limits required by the Chinese Pharmacopoeia for Chinese medicinal materials.The newly developed test strip was compared with LC-MS/MS for the detection of multiple mycotoxins in 85 commercially purchased medicinal materials.The two methods showed identical detection rates,indicating that the technique offers high accuracy,comprehensiveness,sensitivity,and anti-interference capability.Owing to its low cost and high efficiency,the test strip is suitable for large-scale rapid screening in primary-level laboratories,providing technical support for the rapid multi-mycotoxin screening of Chinese medicinal materials.